This bulletin also provides a variety of scenarios you can use to determine which option or combination of options best suits your needs. The purpose of this user bulletin is to clarify these installation options by providing 10 different options, including one for a day demonstration package designed to help you determine if your current computer configuration supports. Installation Options for the Option 1: Install the Day Demo Software Option 2: Install the Full Version of on a Universal Option 6: Install the on a Universal Option 7: Install the Generic Updater Option 8: Install the Full Version of on a Option 9: Install the on a Option Install the Server on a Upgrade Scenarios for The full client-server functionality available in the GeneMapper increases the installation options for Applied Biosystems customers. GeneMapper® Software v to v Server Upgrade. features of Data Collection v Software* on Series provided with the GeneMapper® software. described in the GeneMapper® Software Version Installation and Administration For example: Type 0, then press Enter for each of the remaining fields. GeneMapper® Software Version SNPlex™ System Analysis Getting Started Guide v. After determining the optimal pooling ratio and/or dilution ratio, you can then use the same dilutions for subsequent analyses, as PCR yields should be relatively consistent.5 0 pm, 4. Send in 1 µL of each dilution series to the GUDSF for capillary electrophoresis and sizing. Then carry out a series of dilutions on the pooled reactions in order to determine the optimal fluorescence for running on the 3130xl. The fluorescent dyes are detected with different efficiencies, therefore the amount of each dye-labelled product in the pool will require adjustment to ensure even detection.Ī good way to proceed is to test a few combinations of pooled PCR reactions to determine the pooling ratio that will provide similar fluorescent intensities across all the pooled fragments. If you intend to pool PCR products, it is important to pool PCR products together at the correct ratios in order to get similar fluorescent intensities across all fragments in the pool. Artefact peaks can corrupt both the automated size calling and the analysis of co-loaded samples. This results in artefact “pull-up” peaks that can appear in other colours. Above this range, the instrument cannot measure the true value of the signal and therefore cannot apply the matrix correctly.

For optimal results, the fluorescent signal should be between 150 – 6000 RFUs.

Too much signal is the most common problem. Typically, the required dilution lies in the range from 1:3 – 1:80 ( PCR product:distilled H2O). You will almost always need to dilute PCR amplification products before sending to the GUDSF for analysis. It is the responsibility of the customer to optimise the concentration of reaction products in the sample.

Genemapper software 6